Proximity As Method

Download or read book Proximity Labeling written by Murat Sunbul and published by Humana. This book was released on 2020-08-14 with total page 231 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides detailed protocols and untold tips and tricks regarding the most well-known examples of proximity labeling methods, in which the protein of interest is genetically fused to or labeled with an enzyme that can generate short-lived reactive species to non-specifically label molecules within a certain radius of up to twenty nanometers. Beginning with peroxidase-based proximity labeling methods, the volume continues with BioID, proximity labeling methods that describe the proximity ligation assay to detect RNA-DNA interactions, UV cross-linking to demonstrate RNA-protein interactions, and how chemical and enzymatic reactivities can be improved upon DNA-DNA and protein-protein interactions, as well as “proximity-induced self-labeling,” where the radius of labeling is zero. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Proximity Labeling: Methods and Protocols serves as an ideal guide for researchers exploring the crucial roles that proximity-driven reactions play in biological systems.

Download or read book The Reviewer’s Guide to Quantitative Methods in the Social Sciences written by Gregory R. Hancock and published by Routledge. This book was released on 2018-11-15 with total page 515 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Reviewer’s Guide to Quantitative Methods in the Social Sciences provides evaluators of research manuscripts and proposals in the social and behavioral sciences with the resources they need to read, understand, and assess quantitative work. 35 uniquely structured chapters cover both traditional and emerging methods of quantitative data analysis, which neither junior nor veteran reviewers can be expected to know in detail. The second edition of this valuable resource updates readers on each technique’s key principles, appropriate usage, underlying assumptions and limitations, providing reviewers with the information they need to offer constructive commentary on works they evaluate. Written by methodological and applied scholars, this volume is also an indispensable author’s reference for preparing sound research manuscripts and proposals.

Download or read book Database Systems for Advanced Applications written by Jian Pei and published by Springer. This book was released on 2018-05-11 with total page 845 pages. Available in PDF, EPUB and Kindle. Book excerpt: This two-volume set LNCS 10827 and LNCS 10828 constitutes the refereed proceedings of the 23rd International Conference on Database Systems for Advanced Applications, DASFAA 2018, held in Gold Coast, QLD, Australia, in May 2018. The 83 full papers, 21 short papers, 6 industry papers, and 8 demo papers were carefully selected from a total of 360 submissions. The papers are organized around the following topics: network embedding; recommendation; graph and network processing; social network analytics; sequence and temporal data processing; trajectory and streaming data; RDF and knowledge graphs; text and data mining; medical data mining; security and privacy; search and information retrieval; query processing and optimizations; data quality and crowdsourcing; learning models; multimedia data processing; and distributed computing.

Download or read book Practical Research Methods for Nonprofit and Public Administrators written by Gary R. Rassel and published by Taylor & Francis. This book was released on 2022-12-27 with total page 401 pages. Available in PDF, EPUB and Kindle. Book excerpt: Practical Research Methods for Nonprofit and Public Administrators, Second Edition, covers basic principles of research design, data collection, data analysis, reporting and ethical principles of research. The discussion describes the skills as they would be used in practice in a not-for-profit or public administration environment. The book gives pragmatic instructions for designing research to ensure that results will be accurate and administrators can have confidence in them. It covers techniques to assure that researchers are measuring what they intend to measure and in a way that will be useful and manageable. Sampling methods to find out about larger populations are discussed as are survey, interview, and focus group data collection procedures. The book describes how Geographic Information Systems (GIS) are used to obtain information on the locations of service needs and providers and to draw maps showing these. Participatory research methods including community needs assessments and asset mapping are discussed. The book includes chapters on statistical analysis, visual displays of data, reporting of results and evaluation of programs. The use of computer programs for analyzing data is detailed and illustrated by examples. The appropriate software for analysis and mapping is discussed. A chapter updates the ethics and government requirements for research. Each chapter includes examples and exercises to enhance learning. The book is intended for administrators in not-for-profit and public administration organizations, providing practical skills that can be used in many situations. It is also intended for use as a text in academic programs in administration and management.

Download or read book Software Design X-Rays written by Adam Tornhill and published by Pragmatic Bookshelf. This book was released on 2018-03-08 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Are you working on a codebase where cost overruns, death marches, and heroic fights with legacy code monsters are the norm? Battle these adversaries with novel ways to identify and prioritize technical debt, based on behavioral data from how developers work with code. And that's just for starters. Because good code involves social design, as well as technical design, you can find surprising dependencies between people and code to resolve coordination bottlenecks among teams. Best of all, the techniques build on behavioral data that you already have: your version-control system. Join the fight for better code! Use statistics and data science to uncover both problematic code and the behavioral patterns of the developers who build your software. This combination gives you insights you can't get from the code alone. Use these insights to prioritize refactoring needs, measure their effect, find implicit dependencies between different modules, and automatically create knowledge maps of your system based on actual code contributions. In a radical, much-needed change from common practice, guide organizational decisions with objective data by measuring how well your development teams align with the software architecture. Discover a comprehensive set of practical analysis techniques based on version-control data, where each point is illustrated with a case study from a real-world codebase. Because the techniques are language neutral, you can apply them to your own code no matter what programming language you use. Guide organizational decisions with objective data by measuring how well your development teams align with the software architecture. Apply research findings from social psychology to software development, ensuring you get the tools you need to coach your organization towards better code. If you're an experienced programmer, software architect, or technical manager, you'll get a new perspective that will change how you work with code. What You Need: You don't have to install anything to follow along in the book. TThe case studies in the book use well-known open source projects hosted on GitHub. You'll use CodeScene, a free software analysis tool for open source projects, for the case studies. We also discuss alternative tooling options where they exist.

Download or read book Formal Methods and Software Engineering written by Lindsay Groves and published by Springer. This book was released on 2013-10-21 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book constitutes the refereed proceedings of the 15th International Conference on Formal Engineering Methods, ICFEM 2013, held in Queenstown, New Zealand, in October/November 2013. The 28 revised full papers together with 2 keynote speeches presented were carefully reviewed and selected from 88 submissions. The topics covered are abstraction and refinement, formal specification and modeling, program analysis, software verification, formal methods for software safety, security, reliability and dependability, tool development, integration and experiments involving verified systems, formal methods used in certifying products under international standards, and formal model-based development and code generation.

Download or read book Proceedings of the 2nd International Conference on Advanced Civil Engineering and Smart Structures written by TianQiao Liu and published by Springer Nature. This book was released on with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: